Figure 2

Triptolide protects neuronal cells from the toxicity of LPS-stimulated microglia-conditioned medium. (A) Triptolide attenuated the toxicity of conditioned medium from LPS-stimulated microglia to MN9D cells. MN9D cells treated with conditioned medium from LPS-unstimulated microglia, LPS-stimulated microglia with or without triptolide (50 nM), as well as triptolide alone (50 nM) treated microglia for 24 h. Cell viability was assessed by MTS assay. (B) Triptolide attenuated the toxicity of conditioned medium from LPS-stimulated microglia to SH-SY5Y cells. SH-SY5Y cells treated with conditioned medium from LPS-unstimulated microglia, LPS-stimulated microglia with or without triptolide (50 nM), triptolide alone (50 nM) treated microglia, as well as LPS-stimulated microglia with FeTMPyP (10 μM) for 72 h. Cell viability was assessed by MTS assay. (C) Triptolide did not change primary rat microglial cell viability. Primary rat microglial cells were treated with triptolide (0, 6.25, 12.5, 25, 50 nM) for 24 h. Cell viability was assessed by MTS assay. (D) Triptolide did not change MN9D cell viability. MN9D cells were treated with triptolide (0, 6.25, 12.5, 25, 50 nM) for 24 h. Cell viability was assessed by MTS assay. **P < 0.01. ^# P < 0.05 and ^## P < 0.01.