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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Anti-inflammatory effects of astroglial α7 nicotinic acetylcholine receptors are mediated by inhibition of the NF-κB pathway and activation of the Nrf2 pathway

Fig. 5

α7 nAchR activation induced upregulation of canonical Nrf2 antioxidant genes. a Pretreatment with GTS21 (15 and 30 μm) for 1 h resulted in significant upregulation of canonical Nrf2 antioxidant genes HO1, TXNRD1, and GCLC, in astrocytes treated with LPS for 24 h. α7 nAchR antagonist MLA (1 μm) significantly reduced this effect indicating the specificity of this response. *p < 0.05 compared with LPS, 60 ng/ml treatment, # p < 0.05 compared with GTS21 30 μm + LPS 60 ng/ml (both using one-way ANOVA with adjustment for multiple comparisons using Tukey’s test). Error bars represent SD (n = 4). b A significant increase in protein levels of HO1, NQO1, and TXNRD1 upon GTS21 (30 μm) treatment was noted using western blot in astrocytes treated with LPS for 24 h, and this effect was blocked by the 1 μm α7 nAchR antagonist MLA. *p < 0.05 compared with LPS 60 ng/ml treatment, # p < 0.05 compared with GTS21 30 μm + LPS 60 ng/ml treatment (both using one-way ANOVA with adjustment for multiple comparisons using Tukey’s test). Error bars represent SD (n = 3)

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