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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Anti-inflammatory effects of astroglial α7 nicotinic acetylcholine receptors are mediated by inhibition of the NF-κB pathway and activation of the Nrf2 pathway

Fig. 6

Antioxidant and anti-inflammatory responses of α7 nAchR activation are Nrf2 dependent. a Pretreatment with GTS21 (15 and 30 μm) resulted in significant upregulation of canonical Nrf2 antioxidant genes HO1, TXNRD1, GCLC, and OSGIN1 in wild-type astrocyte cultures treated with LPS (60 ng/ml) for 24 h, but not in astrocytes from Nrf2 knockout mice. *p < 0.05 compared with LPS (60 ng/ml) treatment (one-way ANOVA with adjustment for multiple comparisons using Dunnett’s test). Error bars represent SD (n = 4). b One hour pretreatment with GTS21 (15 and 30 μm) in astrocytes isolated from Nrf2 knockout mice treated with LPS (60 ng/ml) for 24 h resulted in substantially reduced blockage of pro-inflammatory cytokine, TNF-α, secretion (~ 22%) as compared with astrocytes cultured from wild-type mice (~ 63%). *p < 0.05 compared with LPS (60 ng/ml) treatment (one-way ANOVA with adjustment for multiple comparisons using Dunnett’s test). Error bars represent SD (n = 4)

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