Table 4 Sequences of specific primer sets, used for Q-PCR analysis
From: The human microglial HMC3 cell line: where do we stand? A systematic literature review
Genes | Forward primer | Reverse primer | Product length (Base pair) | NCBI Reference sequence | mRNA variants |
|---|---|---|---|---|---|
Lineage markers | |||||
IBA1 | F150–170: TCATGTCCCTGAAACGAATG | R265–285: CCAGCATCATCCTGAGAAAG | 136 bp | NM_032955.2 | 1, 3 and 4 |
CD14 | F636–655: CGCTCGAGGACCTAAAGATA | R711–693: AAGCTGGAAAGTGCAAGTC | 76 bp | NM_001174105.1 | 4 |
CX3CR1 | F187-207: GGCTGAGGCCTGTTATATTG | R315–333: TGACACTCTTGGGCTTCT | 147 bp | NM_001171174.1 | 1, 2, 3 and 4 |
CCR2 | F425–445: CCACAAGCTGAACAGAGAAA | R558–579: GGGAGCACCGTAATCATAATC | 155 bp | NM_001123041.2 | A and B |
P2RY12 | F674–692: AGACCACCAGGCCATTTA | R837–858: CAGACTAGACCGAACTCTGAT | 185 bp | NM_022788.4 | 1 and 2 |
CSF1R | F344–364: CAGGGAATCCCAGTGATAGA | R470–489: TGGAGCCATCAGAGTACAG | 146 bp | NM_005211.3 | 2, 3 and 4 |
TMEM119 | F408–428: ACTTCCTGGATGGGATAGTG | R542–562: GGGAAGGACGATGGGTAATA | 155 bp | NM_181724.2 | |
TREM2 | F588–607: AGCCTCTTGGAAGGAGAAA | R719–737: AGTTCACTGGGTGGATGT | 150 bp | NM_018965.3 | |
Inflammatory genes | |||||
IL-6 | F394–414: CCTTCCAAAGATGGCTGAAA | R 524–543: TGGCTTGTTCCTCACTACT | 150 bp | NM_000600.4 | 1 and 2 |
TNFα | F1305–1324: GAGCCAGCTCCCTCTATTTA | R 1485–1466: GGGAACAGCCTATTGTTCAG | 181 bp | NM_000594.3 | |
IL-1β | F407-427: CATGGGATAACGAGGCTTATG | R 556–537: CCACTTGTTGCTCCATATCC | 150 bp | NM_000576.2 | |
COX2 (Rat/ Human) | F1384–1407:TTGCTGGCAGGGTTGCTGGTGGTA | R1469–1449: CATCTGCCTGCTCTGGTCAATCGAA | 86 bp | NM_000963.4 | |
NOS2 (Rat/ Human) | F 1701–1725: CTGCATGGAACAGTATAAGGCAAAC | R 1929–1905: AGACAGTTTCTGGTCGATGTCATGA | 229 bp | AF049656.1 | |
TGFβ: | F2423-2442: CAGTCACCATAGCAACACTC | R 2583–2567: CCTGGCCTGAACTACTATCT | 161 bp | NM_000660.5 | |
ARG1 | F 905–925: GGGCTACTCTCAGGATTAGAT | R 1016–996: CGAAACAAGCCAAGGTTATTG | 112 bp | NM_001244438.1 | 1 and 2 |
Normalization | |||||
ACTB | F590–613: ACGTTGCTATCCAGGCTGTGCTAT | R830–807: TTAATGTCACGCACGATTTCCCGC | 241 bp | NM_001101.3 | |