Fig. 3
Effects of central CTRP4 overexpression on hypothalamic leptin signaling. (a-c) 60 h after central intervention, mice were fasted for 12 h, then either saline (leptin− group) or leptin (3 μg/mice) (leptin+ group) was i.c.v. injected. Fifteen minutes later, the mice were anesthetized, and hypothalamic tissue was collected for Western blot analysis. (a) Immunoblots showing p-STAT3Tyr705, STAT3, p-JAK2, and β-actin levels in saline- and leptin-stimulated mice fed an SCD (SCS) or HFD (HFS, HFG, and HFC) after central intervention. (b-c) Quantitative analysis of p-STAT3Tyr705 and p-JAK2 expression. (d-e) At 72 h after i.c.v. injection, the mice were anesthetized, and then hypothalamic tissue was collected for SOCS3 protein analysis. (d) Representative immunoblots showing hypothalamic SOCS3 in SCD- and HFD-fed mice that received saline (SCS and HFS), Ad-GFP (HFG), and Ad-CTRP4 (HFC). (e) Quantitative analysis of SOCS3 expression. n = 5 per group. A two-tailed unpaired Student’s t test or the Mann-Whitney test, as appropriate, was used for two-group comparisons. Two-way analysis of variance (ANOVA) and Tukey’s test for multiple comparisons were used to analyze differences among groups. (b-c) ▲P<0.05, ▲▲P<0.01 leptin+ group vs. leptin− group, #P<0.05, ##P<0.01 vs. SCS leptin+ group, *P<0.05, **P<0.01 vs. HFG leptin+ group. (e) #P<0.05, ##P<0.01 vs. SCS group, *P<0.05, **P<0.01 vs. HFG group