Fig. 2

Knockdown of SPHK1 suppressed OGD/R-induced cell death. a MTT assay to analyze cell viability following various periods of OGD/R. b Relative SPHK1 protein level in cells transfected with sh-NC or sh-SPHK1. c MTT assay to analyze cell viability following OGD 8h/R in transfected cells. d TUNEL staining to measure the number of apoptotic cells in transfected cells upon OGD 8h/R. e Relative protein levels of SPHK1 and NF-κB p65 in transfected cells following OGD 8h/R. f Relative levels of TNF-α, IL-6, and IL-1β in transfected cells following OGD 8h/R. All the results were shown as mean ± SD (n = 3), which were three different experiments performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.01