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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Brain gray matter astroglia-specific connexin 43 ablation attenuates spinal cord inflammatory demyelination

Fig. 5

Immunohistochemistry of representative A1 and A2 astroglia markers in GFAP+ astroglia during acute EAE. Double immunofluorescence for C3 (green), an A1 astroglia marker, and GFAP (red) (a, e, i) or S100A10 (green), A2 astroglia markers, and GFAP (red) (c, g, k) in the cerebrum (a, c), the cerebellum (e, g), and the spinal cord (i, k) from Cx43fl/fl and Cx43 icKO mice in the pre-immunized and peak phases (dpi 17) of EAE. Quantitative analysis results of C3/GFAP double-positive (b, f, j) and S100A10/GFAP double-positive areas (d, h, l) in each region of the CNS from Cx43 icKO and Cx43fl/fl mice in the pre-immunized and peak phases (dpi 17) of EAE are shown. All data are presented as the means ± SEM of 4 mice per group. The statistical significance of differences in each comparison was analyzed by one-way ANOVA, followed by Tukey’s post-hoc analysis. All figures were counterstained by DAPI (blue). *P < 0.05, **P < 0.01, ***P < 0.001. NS not significant. Scale bars: 500 μm (i, k), 200 μm (a, g), and 100 μm (c, e)

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