Fig. 4
From: Visceral adipose tissue imparts peripheral macrophage influx into the hypothalamus
Peripheral macrophages that increase in the hypothalamus following HFD express pro-inflammatory markers. A The percent of hypothalamic macrophages defined as CD11b+CD45high that express M2-like marker CD206, M1-like pro-inflammatory marker CD11c+PDL1+, and the marker of adipose tissue, metabolically activated, macrophages (ATM) CD11c+CD36+. Bar graphs present the % of all live cells, while pie charts present their proportion of only macrophages. B, C Visceral fat depot (B) or PEC (C) macrophages defined as F4/80+CD11b+ that contain CD206+, or CD11c+PDL1+, or CD11c+CD36+. Bar graphs represent the % of macrophages with these markers of all live cells, while pie charts present the macrophage populations only. n=9–12 per group. Statistical significance (*) between Ctr (gray bars) and HFD (black bars) were determined by Student’s T test followed by Tukey’s post hoc test