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Fig. 10 | Journal of Neuroinflammation

Fig. 10

From: D-dopachrome tautomerase activates COX2/PGE2 pathway of astrocytes to mediate inflammation following spinal cord injury

Fig. 10

Effects of D-DT inhibition on the production of PGE2 following rat SCI. a Western blot analysis of COX1, COX2, mPGES-1, mPGES-2, and cPGES at 0, 1, 4, and 7 days following injection of 8 μl of 100 mM 4-CPPC inhibitor at lesion sites of the contused cord. b–f Quantification data as shown in (a). Quantities were normalized to endogenous β-actin. g–j Immunostaining of COX2 in the astrocytes following cord treatment with vehicle (g, h) or 4-CPPC inhibitor (i, j) at 4 days. Rectangle indicates region magnified. Arrowheads indicate the positive signals. k ELISA determination of PGE2 following cord treatment with 4-IPP or 4-CPPC inhibitor at 0, 1, 4, and 7 days, respectively. Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05). Scale bars, 500 μm in (g, i); 50 μm in (h) and (j)

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