Fig. 3

Determination of COX2 protein levels and colocalization of COX2 with astrocytes following SCI. a Western blot analysis of COX1, COX2, mPGES-1, mPGES-2, and cPGES at 0, 1, 4, and 7 days following SCI. b–d Quantification data as shown in (a). Quantities were normalized to endogenous β-actin. e–l Immunostaining showed colocalization of COX2 with GFAP- and S100β-positive astrocytes. Rectangle indicates region magnified. Arrowheads indicate the positive signals. Scale bars, 500 μm in (e), (g), (i), and (k); 50 μm in (f), (h), (j), and (l). Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05)