Fig. 4
Determination of PGE2 synthesis-related protein levels following astrocytes incubation with recombinant D-DT protein. a Primary astrocytes stained with GFAP and Hoechst 33342 with the purity over 95%. b Western blot analysis of COX1, COX2, mPGES-1, mPGES-2, and cPGES following astrocyte stimulation with 0, 0.1, 0.5, 2.5 μg/ml recombinant D-DT protein for 24 h. c–e Quantification data as shown in (b). Quantities were normalized to endogenous β-actin. f, g ELISA determination of PGE2 in supernatant and lysate following astrocytes stimulation with 0, 0.1, 0.5, and 2.5 μg/ml recombinant D-DT protein for 24 h. Scale bars, 100 μm in (a). Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05)