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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Early glycolytic reprogramming controls microglial inflammatory activation

Fig. 2

Aerobic glycolysis is required for microglial inflammatory activation. BV-2 microglial cells or primary microglial cells were pretreated with 2-DG (600-1000 μM) for 30 min, followed by LPS (200 ng/mL) treatment for 24 h. The nitrite in the cell culture medium was determined using the Griess reaction (a BV-2 microglial cells; c primary microglia). Cell viability was determined by MTT assay (b BV-2 microglial cells; d primary microglia). The concentrations of IL-6 (e) and TNF-α (f) in the primary microglial cell culture media were analyzed by ELISAs. The expression of iNOS, COX-2, and IL-1β (g) in BV-2 microglial cells was determined by Western blotting (upper), and the relative protein levels were quantified by densitometry analysis (lower). Data are presented as mean ± S.D. (n=3) and are representative of results obtained from three independent experiments. *p < 0.05, **p <0.01, compared to the NONE group or LPS alone group

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