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Table 3 Secondary antibodies used at immunohistochemistry

From: Aging and high-fat diet feeding lead to peripheral insulin resistance and sex-dependent changes in brain of mouse model of tau pathology THY-Tau22

Staining

Secondary antibody

Dilution

Manufacturer

DAB

Peroxidase rabbit IgG

1:400

Vector Lab., Burlingame, CA, USA

Fluorescence

AlexaFluor 488 mouse pAb

1:1000

Thermofisher, Rockford, IL, USA

AlexaFluor 568 rabbit pAb

1:1000

Thermofisher, Rockford, IL, USA

  1. DAB 3,3′-diaminobenzidine
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Journal of Neuroinflammation

ISSN: 1742-2094