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Table 4 Primary antibodies used at western blots of hippocampi

From: Aging and high-fat diet feeding lead to peripheral insulin resistance and sex-dependent changes in brain of mouse model of tau pathology THY-Tau22

Primary antibody

Dilution

Diluent

Manufacturer

β-actin mouse mAb

1:10,000

5% milk

Sigma-Aldrich, St. Louis, MO, USA

Akt rabbit mAb

1:1000

5% BSA

Cell Signaling Technology, Danvers, MA, USA

pAkt (T308) rabbit mAb

1:1000

5% BSA

Cell Signaling Technology, Danvers, MA, USA

pAkt (S473) rabbit mAb

1:1000

5% BSA

Cell Signaling Technology, Danvers, MA, USA

CD11b rabbit mAb

1:1000

5% milk

Abcam, Cambridge, UK

p44/42 ERK1/2 mouse mAb

1:2000

5% milk

Cell Signaling Technology, Danvers, MA, USA

p44/42 pERK1/2 (T202/Y204) mouse mAb

1:2000

5% milk

Cell Signaling Technology, Danvers, MA, USA

GFAP mouse mAb

1:1000

5% milk

Cell Signaling Technology, Danvers, MA, USA

GSK-3β rabbit mAb

1:1000

5% BSA

Cell Signaling Technology, Danvers, MA, USA

pGSK-3β (S9) rabbit mAb

1:1000

5% BSA

Cell Signaling Technology, Danvers, MA, USA

PDK-1 rabbit pAb

1:1000

5% BSA

Cell Signaling Technology, Danvers, MA, USA

pPDK-1 (S241) rabbit mAb

1:1000

5% BSA

Cell Signaling Technology, Danvers, MA, USA

PI3Kp85 rabbit mAb

1:1000

5% BSA

Cell Signaling Technology, Danvers, MA, USA

PP2A C rabbit mAb

1:1000

5% BSA

Cell Signaling Technology, Danvers, MA, USA

methyl-PP2A C (L309) mouse mAb

1:1000

5% milk/ 1% BSA

Millipore, Temecula, CA, USA

pPP2A (Y307) rabbit mAb

1:5000

5% milk

Abcam, Cambridge, UK

PSD95 rabbit pAb

1:1000

5% BSA

Cell Signaling Technology, Danvers, MA, USA

Synaptophysin rabbit pAb

1:5000

5% milk

Santa Cruz Biotechnology, Inc., Dallas, TX, USA

Tau 5 mouse mAb

1:5000

5% milk

Invitrogen Corp., Frederick, MD, USA

pTau (pThr212) rabbit pAb

1:5000

5% BSA

Thermofisher, Rockford, IL, USA

pTau (pSer214) rabbit pAb

1:1000

5% BSA

Thermofisher, Rockford, IL, USA

pTau (pThr231) rabbit pAb

1:10,000

5% BSA

Thermofisher, Rockford, IL, USA

pTau (pSer396) rabbit pAb

1:10,000

5% BSA

Thermofisher, Rockford, IL, USA

pTau (pSer404) rabbit pAb

1:1000

5% BSA

Thermofisher, Rockford, IL, USA

pTau (pSer422) rabbit pAb

1:1000

5% BSA

Thermofisher, Rockford, IL, USA

  1. BSA bovine serum albumin, mAb monoclonal antibody, pAb polyclonal antibody
  2. All diluents are diluted in TBS/Tween-20; non-fat dry milk was used