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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: NLRP3 inhibition attenuates early brain injury and delayed cerebral vasospasm after subarachnoid hemorrhage

Fig. 3

NLRP3 inhibition reduces early brain injury after SAH. A Brain water content measurements of the cerebral cortex 24 h post-SAH, n = 3 per group. B, C Western blot for transmembrane tight junction protein occluding (B) and ZO-1 (C) 24 h post-SAH, n = 4 per group. D Immunofluorescence staining for infiltrating neutrophils (red) 24 h post-SAH with Iba1 co-stain (green) to distinguish microglia in sham (left), SAH + vehicle (middle), and SAH + MCC950 (right) groups with DAPI nuclear counterstain (blue), scale bars = 50μm, red arrowheads indicate individual neutrophils. E Quantification of neutrophils (Ly6G/C+ cells) per HPF, n = 4 per group. F Representative images of fibrinogen-stained (red) cerebral cortex images captured with 40× or 64× (inset) objective lens. G Quantification of microthrombi per high-powered field (40×), n = 5–6 per group. All data presented as mean ± SEM, * p < 0.05, ** p < 0.01, compared to sham surgery group, # p < 0.05 compared to SAH + vehicle group by Kruskal-Wallis test with Dunn’s multiple comparisons test

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