Fig. 4

NLRP3 inhibition reduces neuronal apoptosis 24 h after SAH. A Representative images of TUNEL assay with NeuN co-stain in sham + vehicle (top), SAH + vehicle (middle), and SAH + MCC950 (bottom). B Representative images of TUNEL assay with Iba1 co-stain. C Quantification of total TUNEL-positive cells per high-powered field (HPF). D Quantification of TUNEL-positive neurons as a proportion of total NeuN-positive cells per HPF. E Quantification of TUNEL-positive microglia as a proportion of total Iba1-positive cells per HPF. Images were captured with 10× or 40× objective lens, scale bars = 200μm for 10× images and 50μm for 40× images, n= 4 in each group, * p < 0.05 compared to sham+ vehicle group, ** p < 0.01 compared to sham + vehicle group by Kruskal-Wallis test with Dunn’s multiple comparisons test