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Table 1 Antibodies utilized with source, catalog number, and dilution

From: NLRP3 inhibition attenuates early brain injury and delayed cerebral vasospasm after subarachnoid hemorrhage

Antibody

Source (catalog #)

Dilution (rel. to 1mg/mL stock)

Mouse anti-GAPDH

Abcam (ab8245)

1:10,000

Anti-mouse IgG (HRP-conj.)

Abcam (ab205729)

1:25,000

Anti-rabbit IgG (HRP-conj.)

Abcam (ab6721)

1:25,000

Rabbit anti-NLRP3

Bioss (BS-10021R)

1:2000

Rabbit anti-caspase-1 (p10)

Abcam (ab1795015)

1:1000

Mouse anti-capase-1 (p20)

Adipogen (AG-20B-0042-C100)

1:1000

Rabbit anti-IL-1β

Abcam (ab9722)

1:1000

Rabbit anti-ZO-1

ThermoFisher (61-7300)

1:2000

Mouse anti-occludin

ThermoFisher (OC-3F10)

1:1000

Rabbit anti-Iba1

Wako (019-19741)

1:1000

Mouse anti-Iba1

ThermoFisher (MA5-27726)

1:100

Rabbit anti-NeuN

Millipore Sigma (ABN78)

1:500

Rat anti-NIMP-14 (Ly6G/C)

Abcam (ab2557)

1:200

Rabbit anti-fibrinogen β-chain

ThermoFisher (16747-1-AP)

1:100