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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Microglial responses to CSF1 overexpression do not promote the expansion of other glial lineages

Fig. 2

CSF1 OE does not increase expression of M2 markers, even in aged mice. A Representative images of immunofluorescence for ARG1 (green) with IBA1 (red) in the indicated brain regions in CSF1 OE mice at p14 and 1 year. DAPI (blue) is also shown in the merged image. Arrowheads indicate examples of IBA1+ ARG1− cells while an asterisk indicates an example of lipofuscin autofluorescence that was also visible in additional channels (not shown). B Representative images of immunofluorescence for MRC1 (CD206) (green) with IBA1 (red) in the indicated brain regions in CSF1 OE mice at p14 and 1 year. Arrowheads indicate examples of IBA1+ MRC1− parenchymal microglia and arrows indicate MRC1+ perivascular macrophages residing next to blood vessels that stain with lectin (in white, lectin staining is also present as expected in microglia). DAPI (blue) is also shown in the merged image. Scale bars = 25 μ. RT-qPCR for Arg1 (C) and Mrc1 (D) in p14 and 1-year mice on mRNA isolated from half brain hemispheres of control (CON, white shaded bars) or CSF1 OE (OE, grey shaded bars) mice. ns = non-significant (p > 0.05), unpaired, two-tailed t test

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