Figure 3

Confocal micrographs of MCP-1 receptor, CCR2 and Dcx in the hippocampal DG. (A) As shown in top panels, CCR2-positive cells (green) appeared in the GCL, SGZ and hilus. Within the SGZ, CCR2-positive cells were double-labeled with a neuroblast marker Dcx (red). Note that Dcx-positive cells appeared ectopically within the hilus after SE, and a subset of these cells expressed CCR2. The middle and bottom panels show projected z-stack confocal images to demonstrate colocalization of CCR2 with Dcx. White brackets mark the magnified views of the area. Arrows denote CCR2/Dcx-positive cells. The cell denoted by the asterisk is enlarged to illustrate the pattern of CCR2 and Dcx labeling. Scale bar: top and middle, 50 μm; bottom, 10 μm. GCL: granule cell layer; H: hilus; SGZ: subgranular zone. (B) Negative control immunostaining images for CCR2 when the CCR2 primary antibody was omitted (right) or eliminated by a blocking peptide (left). Neuclei were counterstained with DAPI (blue).