Activation of the inflammatory transcription factor nuclear factor interleukin-6 during inflammatory and psychological stress in the brain

Table 3 Semi-quantitative analysis of LPS-induced nuclear NF-IL6 immunoreactivity

Time (h)	100 μg/kg LPS	1 mg/kg LPS	Control (saline)
Anterior lobe of the pituitary
2	+ (3.0)
4	++ (4.4)
8	+++ (5.0)	+++ (4.6)	± (2.0)
10	++ (4.4)
24	± (2.0)	± (2.4)
Posterior lobe of the pituitary
2	± (2.0)
4	++ (4.1)
8	+++ (4.8)	++ (3.9)	± (2.0)
10	++ (4.2)
24	± (2.0)	± (2.2)

Semi-quantitative analysis of the number of nuclear NF-IL6 signals in the anterior lobe and the posterior lobe of the rat pituitary 2, 4, 8, 10, and 24 h after injection of 100 ug/kg LPS or 8 and 24 h after the injection of 1 mg/kg LPS compared to controls (8 h after injection of saline). A five-point scale was used to rate the data: + + + (5), high density of nuclear signals; + + (4), moderate density of nuclear signals; + (3), low density; ± (2), single nuclear signals in some cases; - (1), no nuclear signals.

ISSN: 1742-2094