Figure 1

Expression of inflammatory cytokine in an acute LPS-treated mouse. (A) Immunohistochemical analysis of the effect of LPS treatment on the microglia in the SNC. PBS (2 μL/injection) or LPS (5 μg/2 μL/injection) was injected into the SNR bilaterally. These representative photomicrographs are coronal sections of the SNC at 6 hr after PBS (upper row) or acute LPS treatment (lower row). Triple immunofluorescence staining was performed with CD11b (green), IL-1β (red) and TH (blue) antibodies. Immunoreactivity for IL-1β was co-localized with that for CD11b after LPS treatment (yellow in the merged photomicrograph). Scale bars: 20 μm (B) Effect of LPS treatment on the gene expression of inflammatory cytokine in the midbrain. The midbrain was obtained 6 hr after the acute LPS treatment. Values are expressed as percentages of those in the PBS-treated group and represented as mean ± SEM. *P < 0.05 compared with the PBS-treated group. IL, interleukin; LPS, lipopolysaccharide; PBS, phosphate-buffered saline; SEM, standard error of the mean; SNC, substantia nigra pars compact; SNR, substantia nigra pars reticulata; Τ Η , tyrosine hydroxylase.