Figure 4

Detection of cell death using Fluoro-Jade B and TH immunostaining. PBS (2 μL/injection, bilaterally) or LPS (5 μg/2 μL/injection, bilaterally) was injected into the SNR daily for 5 consecutive days. (A) The representative photomicrographs of Fluoro-Jade B staining are coronal sections of the SNC 6 hr, 21 days and 30 days after the final PBS (left) or LPS treatment (right). The positive control was prepared after a traumatic brain injury (TBI) in the cortex. (B) The representative photographs of TH immunostaining using diaminobenzidine, are coronal sections of the SN 6 hr after the final PBS (left) or LPS treatment (right). LPS, lipopolysaccharide; PBS, phosphate-buffered saline; SN, substantia nigra; SNC, substantia nigra pars compact; SNR, substantia nigra pars reticulata; Τ Η , tyrosine hydroxylase.