Figure 3

TRIF is important for unilateral tactile allodynia phenotype following L5 SNL. L5 SNL was performed on (A) Myd88 ^-/-, (B) Trif ^lps2, and (C) Myd88 ^-/- /Trif ^lps2 mice. Mice were allowed to recover and were tested at days 7, 9, 12, and 14 post-SNL. The solid black line and dashed line represents the C57BL/6 ipsilateral and contralateral thresholds, respectively. Both (A) Myd88 ^-/- and (C) Myd88 ^-/- /Trif ^lps2 ipsilateral tactile thresholds were significantly different from the C57BL/6 thresholds beginning 7 days post-L5 SNL. Surprisingly, the (B) Trif ^lps2 mice displayed a tactile allodynia in both ipsilateral and contralateral paws. The Trif ^lps2 ipsilateral paw thresholds were not significantly different from the C57BL/6 mice, but the contralateral paws were significantly different beginning at day 12 post-L5 SNL. Data are expressed as mean ± SEM (n = 5–8 mice/group) and analyzed via 2-way ANOVA, followed by Bonferroni post hoc test to compare each time point to the respective C57BL/6 group, ipsilateral or contralateral (*P <0.05 or **P <0.01 for ipsilateral group; ##P <0.01 for contralateral group).