Figure 5

Minocycline (Mino) treatment did not prevent cell death following ischemia-reperfusion (IR). (A and B) Mino was delivered as twice-daily intraperitoneal (IP) injections, with two initial dosages of 45 mg/kg prior to ischemia and dosages of 22.5 mg/kg just prior to ischemia and every 12 h for the next 2 d during the reperfusion period as described in Materials and Methods. One eye of each rat was subjected to IR for 45 min or needle puncture only (Sham) and after 48 h of reperfusion retinas were assayed for (A) caspase-3 activity and (B) DNA fragmentation cell death ELISA. (C) Mino dose–response. Rats were IP injected twice daily with PBS, 2.5 mg/kg (Mino-5), 7.5 mg/kg (Mino-15) or 22.5 mg/kg (Mino-45) beginning 24 h previous to ischemia, and 48 h following IR retinas were assayed by DNA fragmentation cell death ELISA. Results shown are the means and standard error of means obtained from eight animals per group. *P ≤0.05, **P ≤0.01 and ***P ≤0.001 by Student’s t-test.