Figure 13
From: Glimepiride reduces CD14 expression and cytokine secretion from macrophages
Glimepiride reduces the translocation of Toll-like receptor (TLR)-4 to rafts. (A) The % TLR-4 in rafts of RAW 264 cells pre-treated with control medium, 5 μM glimepiride or 5 μM glipizide and incubated with control medium (□) or 50 μM PrP82-146 (■). Values are means ± SD from triplicate experiments performed 4 times, n = 12. *TLR-4 significantly less than those of control cells incubated with PrP82-146. (B) The % TLR-4 in rafts of RAW 264 cells pre-treated with control medium, 5 μM glimepiride or 5 μM glipizide and incubated with control medium (□) or 50 μM Aβ1–42 (■). Values are means ± SD from triplicate experiments performed 4 times, n = 12. *TLR-4 significantly less than those of control cells incubated with Aβ1–42. (C) The % TLR-4 in rafts of RAW 264 cells pre-treated with control medium, 5 μM glimepiride or 5 μM glipizide and incubated with control medium (□) or 50 μM αSN (■). Values are means ± SD from triplicate experiments performed 4 times, n = 12. *TLR-4 significantly less than those of control cells incubated with αSN. (D) The % TLR-4 in rafts of RAW 264 cells pre-treated with control medium, 5 μM glimepiride or 5 μM glipizide as shown and incubated with control medium (□) or 10 ng/ml LPS (■). Values are means ± SD from triplicate experiments performed 4 times, n = 12. *TLR-4 significantly less than those of control cells incubated with lipopolysaccharide (LPS).