Macrophagic and microglial responses after focal traumatic brain injury in the female rat

Table 1 Antibody studies of M1 versus M2 phenotypes in neurotrauma ^a

Study	Injury model	Species	Sex	Methods	Cell type antibodies	M1 markers	M2 markers
Bedi et al. [40]	CCI	Mouse	Male	FC	CD11b, CD45	CD16/CD32	CD206
Hsieh et al. [34]	CCI	Mouse	Male	FC, qRT-PCR on FC-sorted cells	CD11b, CD45, F4/F80, Ly6G	CD86, MHC II, YFP-IL-12p40	YFP-Arg1
Kigerl et al. [9]	SCI	Mouse	Not specified	IHC, qRT-PCR	None	CD16/CD32, CD86, iNOS, MHC II	Arg1, CD206
Kumar et al. [15]	CCI	Mouse	Male	IHC, qRT-PCR	CD11b, CD68, Iba1	MHC II	Ym1
Lagraoui et al. [32]	CCI	Mouse	Male	qRT-PCR, multiplex	None	None	None
Present study	CCI	Rat	Female	FC, IHC, qRT-PCR, multiplex	CD68, Iba1	CD40, CD86	CD163
Wang et al. [35]	CCI	Mouse	Male	IHC, qRT-PCR	Iba1	CD16/CD32	CD206
Zhang et al. [36]	CCI	Rat	Not specified	IHC	CD68	None	CD163

^a Arg1, Arginine 1; CCI, Controlled cortical impact; FC = Flow cytometry; Iba1, Ionized calcium-binding adapter molecule 1; IHC, Immunohistochemistry; IL, Interleukin; iNOS, Inducible nitric oxide synthase; MHC II, Major histocompatibility complex class II; qRT-PCR, Quantitative RT-PCR; SCI = Spinal cord injury, YFP = Yellow fluorescent protein.

ISSN: 1742-2094