Figure 1
Dexamethasone for improved pDNA-IL-10 uptake does not create robust pain reversal. (A and B) animals developed stable allodynia from day 3 to day 10 after chronic constriction injury (CCI), compared to sham-operated animals (ipsilateral, F(1, 20) = 53.54; P < 0.0001; contralateral, F(1, 20) = 71.70; P < 0.0001). On day 10 following CCI surgery, animals received an i.t. injection of dexamethasone (DEX) (62.4 ng, n = 6 or 6.2 ng, n = 6), or equivolume i.t. saline (n = 7) and sham-operated animals received i.t. equivolume saline (n = 6). Three days later, an i.t. injection of pDNA-IL-10 (25 μg) or equivolume saline was given. I.t. pDNA-IL-10 following a pre-injection injection of DEX (62.4 ng) revealed a delayed and partial bilateral pain reversal (ipsilateral, F3,140 = 33.83; P < 0.0001; contralateral, F3,140 = 19.7; P < 0.0001). Black arrows indicate i.t. injections.