Figure 6

Dexamethasone prevents pathogenic Aβ-induced degradation of HCSM cell α actin. Near confluent cultures of HCSM cells were incubated for six days in the presence or absence of 25 μM Dutch mutant Aβ40 with or without daily administration of anti-inflammatory drug (1 μM final concentration). The HCSM cells were extensively washed, solubilized, subjected to SDS-PAGE, and subsequently analyzed by quantitative immunoblotting using mAb1A4 to smooth muscle cell α actin. Two or three separate experiments were performed in triplicate for each tested condition. A representative immunoblot is shown.