Figure 4

Morphine effect on CCL2 production by human neurons. Immunocytochemical staining of highly purified (>95%) human neurons incubated with (A) medium and (B) morphine (10^-4 M) for 24 h was performed with (a) MAP2 antibody (neuronal marker, green), (b) CCL2 antibody (red), and (c) nuclear DAPI stain (blue). Colocalization of MAP2 and CCL2 is shown in the merged images (d).