Figure 5
Effect of MOR antagonist on morphine-mediated stimulation of neuronal CCL2 production. Highly enriched neuronal cultures were pretreated with β-FNA (3 × 10-6 M) for 30 min prior to morphine (10-6 M) treatment for 24 h. Supernatants were collected for CCL2 ELISA. Data are mean ± SEM of triplicates of three separate experiments using neurons derived from different brain specimens. The saturated two-way ANOVA model fit the data well with a R2 of .91 and Levene's test showed no inequality of the group-wise variances (p = .36). The mean inhibitory effect of β-FNA was estimated to be 3.4 with 95% confidence interval (1.2, 5.6). **P < 0.01 versus control; ††P < 0.01 versus morphine treatment.