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Table 1 Rotenone and 3-NP do not affect cell viability

From: Effects of mitochondrial dysfunction on the immunological properties of microglia

 

WST-1 (% of basal)

Condition

Control

LPS

IL-4

LPS + IL-4

Control

100

174 ± 24

121 ± 19

156 ± 24

0.3 mM 3-NP

96 ± 24

158 ± 23

89 ± 24

132 ± 29

1 mM 3-NP

76 ± 25

141 ± 42

107 ± 31

115 ± 29

0.02 μM Rotenone

146 ± 12

194 ± 42

118 ± 21

132 ± 26

0.2 μM Rotenone

115 ± 21

115 ± 38

114 ± 33

111 ± 27

  1. Microglial cells were treated with rotenone or 3-NP and stimulated with 10 ng/ml IL-4. After 24 hrs, 1 μg/ml LPS was added for 18 hrs. The WST-1 assay was performed as described in Methods. Values are mean ± SEM for independent toxicity measurements. n = 6 wells (i.e., 3 separate experiments performed in duplicate); not significantly different from control (ANOVA followed by Dunnett's Multiple Comparison test).
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Journal of Neuroinflammation

ISSN: 1742-2094