Fig. 4

Anti-TNF treatment decreases inflammation during progressive EAE. a Total numbers of CD45^hi inflammatory cells in the CNS at d30 p.i. of WT and GFAPγR1∆ mice treated with α-TNF or α-βgal mAb. b Frequencies of CD8⁺ and CD4⁺ T cells as well as CD11b⁺ myeloid cells within CD45^hi infiltrating cells in the CNS of the three mouse groups as depicted. c Total numbers of Th1 (IFN-γ-producing) and Th17 (IL-17 producing) CD4⁺ T cells derived from the CNS (d30 p.i.) assessed after PMA and ionomycin stimulation. d IFN-γ and IL-17 in supernatants of splenocytes from immunized mice (d30 p.i.) stimulated with MOG^35–55 peptide as determined by ELISA. Data represent the mean values and ± SEM of three separate experiments with five mice per group per experiment. P values determined by Student’s t test