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Fig. 9 | Journal of Neuroinflammation

Fig. 9

From: Microglia-derived IL-1β contributes to axon development disorders and synaptic deficit through p38-MAPK signal pathway in septic neonatal rats

Fig. 9

IL-1β impedes the protein expression of NFM, NFL and synaptophysin in vitro. Panel (a) shows NFM (160 kDa), NFL (68 kDa) and synaptophysin (38 kDa) immunoreactive bands. Bar graphs in (b, c, and d) show changes in the optical density of NFM, NFL and synaptophysin, respectively, following IL-1β administration. The protein expression of NFM, NFL and synaptophysin is significantly reduced in neuron culture at 24 h after IL-1β administration when compared with the corresponding controls. IL-1R antagonist reversed the reduction. (*P < 0.05, **P < 0.01). Immunofluorescene images show the expression of NFM in neuron culture at 24 h after IL-1β (f), IL-1β + IL-1Ra (g), or IL-1Ra (h) administration when compared with the control (e). Scale bars: e–h 100 μm

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Journal of Neuroinflammation

ISSN: 1742-2094