Fig. 6
From: Toll-like receptor 4 in glial inflammatory responses to air pollution in vitro and in vivo
TLR4 response in mixed glia. Mixed glia: TLR4 siRNA treatment lowered TLR4 mRNA and protein, and attenuated nPM induction of TLR4 mRNA. Media groups are non-transfected. a TLR4 mRNA, but not protein, was induced by nPM. TLR4 siRNA decreased TLR4 mRNA levels by 90% in nPM (ANOVA, p < 0.0001) and 70% in control groups (p < 0.0001). LPS treatment reduced TLR4 mRNA (p < 0.05). b TLR4 protein was not changed by nPM or LPS. TLR4 siRNA reduced protein levels by ca. 60% in all groups (ANOVA, p < 0.05)