Table 1 Primers used in real-time qRT-PCR reactions
Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
NLRP3 | CGGTGACCTTGTGTGTGCTT | TCATGTCCTGAGCCATGGAAG |
ASC | GACAGTACCAGGCAGTTCGT | AGTAGGGCTGTGTTTGCCTC |
Caspase-1 | GAACAAAGAAGGTGGCGCAT | AGACGTGTACGAGTGGGTGT |
IL-1beta | CCTATGTCTTGCCCGTGGAG | CACACACTAGCAGGTCGTCA |
IL-18 | ACCACTTTGGCAGACTTCACT | ACACAGGCGGGTTTCTTTTG |
Nqo1 | GTTTGCCTGGCTTGCTTTCA | ACAGCCGTGGCAGAACTATC |
HO-1 | GGTGATGGCCTCCTTGTACC | GTGGGGCATAGACTGGGTTC |
Actin | TCAGCAAGCAGGAGTACGATG | GTGTAAAACGCAGCTCAGTAACA |