Fig. 5

Effect of Bindarit and RS504393 on ethanol-induced inflammation in the developing brain and SIM-A9 cells. a Bindarit (100 mg/kg) or RS504393 (1 mg/kg) was administered by two subcutaneous (SC) injections at 24 and 0.5 h prior to EtOH exposure as described in the “Methods” section. 8 h after EtOH injection, the pups were sacrificed and the brains were harvested. The relative expression for proinflammatory cytokines IL-6 and TNFα in the brain of PD4 mice were determined with IB and quantified as described above. Each data point was the mean ± SEM of six animals. *p ≤ 0.05, statistically significant difference from control group; ^#p ≤ 0.05, statistically significant difference from EtOH-treated group. b SIM-A9 cells were pretreated with Bindarit (300 μM) or RS504393 (100 μM) for 12 h prior to EtOH (0.4%) exposure. After ethanol exposure for 12 h, cells were harvested and processed for the expression of proinflammatory IL-6 and TNFα by IB as described above. Data are the mean ± SEM of three independent experiments. *p < 0.05, statistically significant difference from control group; ^#p < 0.05, statistically significant difference from EtOH-treated group