Fig. 2
From: Cellular source of hypothalamic macrophage accumulation in diet-induced obesity
Parabiosis experiment indicating no recruitment of circulating LysMGFP myeloid cells to the hypothalamus during 12-week HFD feeding. a, b Representative images of parabiosis between LysMGFP and C57 mice and FACS analysis data confirming successful parabiosis. The percentages indicate the proportion of LysMGFP cells among the peripheral blood monocytes. c Confocal images of GFP and DAPI immunostaining in the choroid plexus and hypothalamus of LysMGFP and C57 parabionts showing that circulating LysMGFP cells had infiltrated the choroid plexus but not the hypothalamus of the C57 mice. Scale bars, 100 μm. ARC, hypothalamic arcuate nucleus; D3V, dorsal third ventricle; ME, median eminence; 3V, third ventricle