Fig. 7

Effects of IL-1R inhibition (using rytvela and Kineret) on BMDM-induced photoreceptor toxicity. a An illustration of the experimental design used to evaluate the effects of isolated murine bone marrow-derived MPs (BMDMs) stimulated with LPS/ATP (stimulant of IL-1β secretion). b TUNEL-stained retinal flat mounts cultured in contact with BMDMs for 18 h in the presence or absence of rytvela or Kineret. Scale bar 50 μm. The graph represents the quantification of TUNEL-positive nuclei in the ONL of retinal flat mounts. Data are expressed as mean ± SEM and analyzed by one-way ANOVA with Holm-Sidak correction for multiple comparisons; n = 5–6 per group. ***p < 0.001. c TUNEL-stained retinal flat mounts cultured with the conditioned medium of LPS/ATP-activated or not BMDMs in the presence or absence of rytvela, Kineret, or an anti-IL-1β antibody. Scale bar 50 μm. The graph represents the quantification of TUNEL-positive nuclei in the ONL of retinal flat mounts. Data are expressed as mean ± SEM and analyzed by one-way ANOVA with Holm-Sidak correction for multiple comparisons; n = 3–5 per group. **p < 0.01. d ELISA measurement of IL-1β in the conditioned medium derived from BMDMs treated or not with LPS + ATP. The conditioned medium was incubated with retinal explants for 18 h in the presence of vehicle, rytvela, or Kineret. n = 4–5 per group. ND: not detected