Table 1 Antibody conditions for tissue staining
Ab name | Species | Company | Secondary antibody | Company |
|---|---|---|---|---|
Collagen-1 | Rabbit | Abcam: ab34710 | 594 Donkey anti-rabbit or 488 donkey anti-rabbit | Jackson: 711-585-152 Jackson: 712-545-150 |
F4/80 | Rat | Bio-Rad: MCA497RT | 594 Donkey anti-rat IgG or 488 donkey anti-rat | Jackson: 712-585-150 Jackson: 712-545-150 |
α-SMA | Mouse | Sigma-Aldrich: C6198 | -Cy3 conjugated | – |
Iba-1 | Rabbit | Wako: 013-2769 | 488 Donkey anti-rabbit | Jackson: 712-545-150 |
C3a | Rat | BD Biosciences: 558251 | Goat anti-rat-594 | Invitrogen: A11007 |
C3aR | Chicken | Peninsula Laboratories International: T-2301 | Biotinylated anti-chicken followed by streptavidin 488 | Jackson: 703-065-155 ThermoFisher: S11223 |
Biotinylated Isolectin-B4 | – | VectorLabs: B-1205 | Streptavidin-488 | ThermoFisher: S11223 |
TGF-β | Rabbit | Abcam: ab92486 | 594 Donkey anti-rabbit | Jackson: 711-585-152 |