Fig. 6

The insulin sensitizer rosiglitazone prevents RD-induced cone loss. a Quantitative RT-PCR of Ccl2, Il-6, and Ifn-γ mRNAs normalized to S26 mRNA in mouse retina without RD and 4 days after RD and treatment with rosiglitazone or its vehicle (n = 6–10/group; one-way Anova/Bonferroni test *p = 0.0494, **p = 0.0326, and ***p = 0.0036 versus the control group, ^$p = 0.0028 versus the DMSO group). b Representative images of peanut agglutinine- (PNA, red), cone arrestin- (CAR, green), and ionized calcium-binding adapter molecule 1- (IBA-1, white) stained retinal flatmounts of C57BL6/J mice 7 days after RD and treatment with rosiglitazone or its vehicle (DMSO). Quantification of c of subretinal IBA-1+ cells, d PNA+ cones, and e CAR+ cones in retinal flatmounts of C57BL6/J mice without RD and 7 days after RD and treatment with rosiglitazone or its vehicle (n = 6–12/group; c one-way Anova *p < 0.0001 and ^$p < 0.0001 versus the control group; d one-way Anova/Bonferroni test *p < 0.0001 versus the control group, ^$p = 0.0018 versus the DMSO group; e one-way Anova/Bonferroni test *p < 0.0001 versus the control group, ^$p = 0.0031 versus the DMSO group). f Representative images of peanut agglutinine- (PNA, red), cone arrestin- (CAR, green), and ionized calcium-binding adapter molecule 1- (IBA-1, white) stained retinal flatmounts of C57BL6/J mice 7 days after RD without or with subretinal injection of metformin. Quantification of g subretinal IBA-1+ cells, h PNA+ cones, and i CAR+ cones in retinal flatmounts of C57BL6/J mice without RD and 7 days after RD without or with subretinal injection of metformin (n = 6–12/group; g one-way Anova *p < 0.0001 versus the control group, ^$p < 0.0001 versus the PBS group; h one-way Anova *p < 0.0001 versus the control group, ^$p = 0.0056 versus the PBS group; i one-way Anova *p < 0.0001 versus the control group, ^$p = 0.0004 versus the PBS group). RD, retinal detachment; DMSO, dimethyl sulfoxyde; RGZ, rosiglitazone; PNA, peanut agglutinine; CAR, cone arrestin; IBA-1, ionized calcium-binding adapter molecule 1. Scale bar (a, b, f) = 50 μm. All values are reported as mean ± SEM