Fig. 6

Effects of pharmacological mGlu3 receptor modulation and Grm3 knock-down on microglial Ccl9 expression in vitro. a, c mRNA expression of Ccl9 under the pro-inflammatory condition (IL-1β + IFNγ) in the presence of LY 379268 (1 μM) + Ro 64-5229 (25 μM) in CTRL and LPD/IL-1β cultured microglia at P4 (a) and at P7 (c). Data (mean ± SEM) are relative to the gene expression under basal CTRL conditions. Two-way ANOVA followed by the Newman-Keuls multiple comparison; *p < 0.05, ***p < 0.001 effect of LY 379268 + Ro 64-5229; ^$p < 0.05, ^$$p < 0.01, ^$$$p < 0.001 effect of IL-1β + IFNγ; ^#p < 0.05, ^###p < 0.001 effect of LPD/IL-1β. b, d mRNA expression of Ccl9 in microglial cells sorted from P4 (b) and P7 (d) rat pups cultured under basal or pro-inflammatory (IL-1β + INFγ) conditions ± LY 2389575 (5 μM). The data (mean ± SEM) are relative to the gene expression under basal CTRL conditions. One-way ANOVA followed by the Newman-Keuls multiple comparison test; **p < 0.01, ***p < 0.001, ****p < 0.0001 effect of LY 2389575; ^$p < 0.05 effect of IL-1β + INFγ. e Ccl9 mRNA expression in response to SCR and Grm3 siRNA (50 nM). Data (mean ± SEM) are relative to the mRNA expression for the respective SCR. Unpaired t test; ****p < 0.0001. f Pearson’s correlation between Grm3 expression and Ccl9 48 h after transfection with siGrm3 (50 nM)