Fig 2.

Spinal cord injury induces leukocyte mobilisation in the periphery. Female C57BL/6 mice underwent either a 70kD SCI (black bars, n=4-5/time point) or sham surgery (white bars, n=5/time point). Tissue was collected at 2, 6, 12 and 24 hours post-operation and was compared to naïve baseline (grey bar and dotted line, n=5). Neutrophil mobilisation in the blood (a) and spleen (b), and monocyte mobilisation in the blood (c) and spleen (d) was determined by flow cytometry. Neutrophil (e) and macrophage (f) density in the liver was measured by immunohistochemistry. Representative images of neutrophil and macrophage density in each injury group at peak immune cell density; neutrophil and macrophage density were greatest at 2 and 24 hours post-SCI respectively. Scale bar represents 50μm. Data are presented as mean ±SEM and were analysed by two-way ANOVA, *p<0.05, **p<0.01 with post-hoc test