Fig. 1

Induction of lncRNA in microglia in response to inflammatory stimuli. a Induction of lncRNA in cultured murine microglia (BV2 cells) following stimulation with LPS. BV2 cells were stimulated with 10 μg/ml LPS for 6 h. b Induction of lncRNA in primary murine microglia following stimulation with LPS. Primary microglia were stimulated with 10 μg/ml LPS for 6 h. c Induction of lncRNA in vivo following stimulation with LPS. C57BL/6 J mice were stimulated with 1 mg/kg LPS or equal volume DPBS for 24 h. RNA was isolated from the cortex region of the brains. d Dose response of Nostrill in response to increasing concentrations of LPS. BV2 cells were stimulated with 0.1, 1, or 10 μg/ml LPS for 6 h. e Temporal expression of Nostrill in response to LPS. BV2 cells were stimulated with 10 μg/ml LPS for 2, 6, or 24 h. Unstimulated BV2 cells served as controls for each time point. f Time-course induction of Nostrill in response to stimulation by inflammatory or anti-inflammatory stimuli. BV2 cells were stimulated with IL-4 (20 ng/ml), LPS (10 μg/ml), TNF-α (20 ng/ml), Poly(I:C) (10 μg/ml), or IFN-γ (20 ng/ml) for 2, 6, or 24 h. Unstimulated BV2 cells served as controls for each time point. Data represent means ± SEM from three independent experiments. Expression levels were validated by real-time quantitative PCR. Gapdh was used as a reference gene for normalization. *p < 0.05, **p < 0.01, and ***p < 0.001 vs control. †p < 0.05, and ††p < 0.01 between indicated groups