Fig. 3

Effect of Nostrill induction on expression of inflammatory genes in microglial cells following LPS stimulation. a Validation of knockdown and overexpression of Nostrill in microglial cells. For knockdown, BV2 cells were treated with the designed siRNA to Nostrill for 24 h and subsequently stimulated with LPS (10 μg/ml) for 6 h. A non-specific siRNA sequence was used as the control. For overexpression, BV2 cells were transfected with the PTarget-Nostrill vector for 24 h and then stimulated with LPS (10 μg/ml) for 6 h. BV2 cells treated with the empty PTarget vector were used as the control. Expression levels of selected inflammatory genes, b Arg1, c IL-6, d IL-1β, e TNF-α, f Ccl2, g iNOS, were quantified by using real-time PCR. Gapdh was used as a reference gene for normalization. Data represent means ± SEM from three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001 vs control siRNA. #p < 0.05, ##p < 0.01, and ###p < 0.001 vs empty vector. †p < 0.05, ††p < 0.01, and †††p < 0.001 between indicated groups