Fig. 5

Effect of Nostrill induction on production of nitric oxide in microglia following LPS stimulation. a Dose response of iNOS in response to increasing concentrations of LPS. BV2 cells were stimulated with 0.1, 1, or 10 μg/ml LPS for 6 h. Expression levels were validated by real-time quantitative PCR. Gapdh was used as a reference gene for normalization. b Production of nitric oxide in BV2 cells following knockdown or overexpression of Nostrill. BV2 cells were transfected with the designed siRNA to Nostrill, a scrambled siRNA-control, the PTarget-Nostrill expression vector, or the empty PTarget vector for 24 h. Cells were stimulated with LPS (10 μg/ml) for 6 h. Media was collected and the Griess assay was performed. c Production of nitric oxide in primary murine microglia following knockdown of Nostrill. Primary murine microglia were transfected with the designed siRNA targeting Nostrill or a control scrambled siRNA for 24 h, then stimulated with LPS (10 μg/ml) for 6 h. Media was collected and the Griess assay was performed. Data represent means ± SEM from three independent experiments. ●●●p < 0.001 vs control. *p < 0.05, **p < 0.01, and ***p < 0.001 vs control siRNA. #p < 0.05, ##p < 0.01, and ###p < 0.001 vs empty vector. †p < 0.05, ††p < 0.01, and †††p < 0.001 between indicated groups