Fig. 5

Immunofluorescence of LC-NE neuron and NE detection in the brain and spinal cord. a TH staining shows TH⁺ LC-NE neurons and fibers. b TH⁺ neurons were counted manually, and data indicated that LC-NE neurons in WT mice are gradually lost with aging, although the neuron number of 24-month-old WT mice was not significantly less than that of 3-month-old and 12-month-old WT mice. In 12-month-old APP/PS1 mice, this number decreased significantly compared to that of the 12-month-old WT mice. Three brain slices from each mouse were analyzed and averaged. n = 6. ns, not significant. **P < 0.01. #P < 0.01 compared with 12-month-old APP/PS1 mice. 4V, fourth ventricle. c NE level in the brain and spinal cord of APP/PS1 and WT mice was detected with ELISA. No difference was detected in brain or spinal cord between groups. n = 6, ns, not significant