Fig. 1
From: Senescent accelerated prone 8 (SAMP8) mice as a model of age dependent neuroinflammation
Analysis of PAS accumulations in SAM mice associated with aging and neurodegeneration. (a) Representative captures of hippocampal regions in SAMR1 and SAMP8 mice of 2 and 10 months (m) and 10 m old APP-PS1 mice, showing PAS-positive granules. Brain sections were stained by Schiff’s reagent (red-pink) and nuclei with hematoxylin (blue-brown). Images of coronal sections of cryostat (30 μm thick) were obtained with a ×20 objective (upper images), ×40 and ×100 (from left to right for magnified pictures), with a Nikon Eclipse 50i H550S light microscope and processed with the help of the NIS elements of software and ImageJ. (b) (i) Schematic representation of the regions analyzed. (ii) Quantification of PAS positive accumulates/mm2. SAMR1 is shown in green, SAMP8 in red, and APP PS1 in black. Two months data are represented with triangles and 10 months with circles. **p < 0.01 and ***p < 0.001 with respect to the PAS/mm2 accumulations (n = 3 to 5 males). Scale bars are included in the images