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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: X chromosome escapee genes are involved in ischemic sexual dimorphism through epigenetic modification of inflammatory signals

Fig. 6

Manipulation of KDM impacts IRF expression in neonatal microglia cultures. a–d ChIP analyses show the binding ratio of irf4 DNA with H3K4-me1 over H3K4-me3 after KDM5C siRNA or lenti-KDM5C treatment in both male and female microglia. e–h The binding ratio of irf5 DNA with H3K27-me1 over H3K27-me3 after KDM6A siRNA or lenti-KDM6A treatment by the ChIP assay. i–l irf4 mRNA levels were measured by RT-PCR after KDM5C siRNA or lenti-KDM5C treatment. m–p irf5 mRNA levels were measured by RT-PCR after KDM6A siRNA or lentivirus treatment. Data were averaged from 3 to 4 independent experiments with triplicate wells. *p < 0.05; **p < 0.01; ***p < 0.001 vs. control groups

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Journal of Neuroinflammation

ISSN: 1742-2094