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Table 3 Primers used for qRT-PCR

From: The CD200R1 microglial inhibitory receptor as a therapeutic target in the MPTP model of Parkinson’s disease

Species: Mus musculus

Target mRNA

Accession number

Forward primer (5′➔3′)

Reverse primer (5′➔3′)

Arg1

NM_007482.3

TTGCGAGACGTAGACCCTGG

CAAAGCTCAGGTGAATCGGC

Cd200full

NM_010818.3

GGGCATGGCAGCAGTAGCG

TGTGCAGCGCCTTTCTTTC

Cd200tr

NM_001358443.1

GATGGGCAGTCTGTGGAAGTG

GAGAACATCGTAAGGATGCAGTTG

Cd200R1

NM_021325.3

AGGAGGATGAAATGCAGCCTTA

TGCCTCCACCTTAGTCACAGTATC

COX2

NM_011198.4

TGCAGAATTGAAAGCCCTCT

CCCCAAAGATAGCATCTGGA

gp91phox

NM_007807.5

ACTCCTTGGGTCAGCACTGGCT

GCAACACGCACTGGAACCCCT

IL10

NM_010548.2

TGAATTCCCTGGGTGAGAAG

ACACCTTGGTCTTGGAGCTT

IL1β

NM_008361.4

TGGTGTGTGACGTTCCCATTA

CAGCACGAGGCTTTTTTGTTG

IL6

NM_031168.2

CCAGTTTGGTAGCATCCATC

CCGGAGAGGAGACTTCACAG

iNOS

NM_010927.3

GGCAGCCTGTGAGACCTTTG

GCATTGGAAGTGAAGCGTTTC

MR

NM_008625.2

TCTTTTACGAGAAGTTGGGGTCAG

ATCATTCCGTTCACCAGAGGG

Nrf2

NM_010902.4

GATCCGCCAGCTACTCCCAGGTTG

CAGGGCAAGCGACTCATGGTCATC

TGFβ

NM_011577.2

TGCGCTTGCAGAGATTAAAA

AGCCCTGTATTCCGTCTCCT

TNFα

NM_013693.3

TGATCCGCGACGTGGAA

ACCGCCTGGAGTTCTGGAA

Reference genes

βactin

NM_007393.5

CAACGAGCGGTTCCGATG

GCCACAGGATTCCATACCCA

Gapdh

NM_008084.3

GGTGAAGGTCGGTGTGAACG

CTCGCTCCTGGAAGATGGTG

  1. Arg1, arginase 1; CD200full, full-length CD200; CD200tr, truncated CD200; CD200R1, CD200 receptor 1; COX2, cyclooxygenase 2; Gapdh, glyceraldehyde-3-phosphate dehydrogenase; gp91phox, catalytic subunit of NADPH oxidase; IL10, interleukin 10; IL1β, interleukin 1β; IL6, interleukin 6; iNOS, inducible nitric oxide synthase; MR, mannose receptor; Nrf2, nuclear factor erythroid 2-related factor 2; TGFβ, transforming growth factor β; TNFα, tumour necrosis factor α
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Journal of Neuroinflammation

ISSN: 1742-2094