Fig. 5

CPE observed in weanling BCECs associated with active caspase-3. Weanling and adult BCECs isolated from OB and cortex were infected with LACV. At 48 hpi, the samples were stained for LACV (green), active caspase-3 (red), and nuclear counterstain DAPI (blue). Scale bar represents 100 μm. BCECs were mock-infected (a, e, i, m) or infected with LACV with an MOI of 0.1 (b, f, j, n), 1 (c, g, k, o), and 10 (d, h, l and p). Weanling OB (a–d), adult OB weanling cortical (e–h), weanling coritcal (i–l), and adult cortical (m–p) BCECs are shown. Arrows indicate active caspase-3 staining in and around LACV-infected cells (b, c, d, j, k, l) or regions of cell loss (d). Relative active caspase-3 presence per cell was quantified by calculating the active caspase-3 sum intensity-to-DAPI area ratio from several randomized from a single experiment set that was used for setting the background fluorescence and image quantification of all group. Quantification of each datapoint is shown as a single symbol along with the mean (orange bar: mean of weanlings and green bar: mean of adults) for each group (q, r). Multiple unpaired t-tests were performed for statistical analyses between matched groups and significance is represented by *P < 0.05 and **P < 0.01